Search results for "DNA analysis"
showing 6 items of 6 documents
Analisi del DNA genomico nucleare di popolamenti a Cystoseira spp. nell’Area Marina Protetta di Capo Gallo-Isola delle Femmine (Sicilia nord-occident…
2008
In this preliminary study we report the first observations on nuclear DNA analysis of Cystoseira populations at Capo Gallo - Isola delle Femmine MPA (north-western Sicily). Some specific bands resulted from the DNA extraction of 2 species even though aspecific bands, probably due to polysaccarides and microendophytes were also highlighted.
A new system for animal products traceability and authentication: Use of DNA analysis of natural tracers and example of application to dry cured hams
2010
A few DNA based approaches have been developed to trace animal products from the farm to the consumer “fork”. These approaches make use of the animal DNA that can be recovered during all steps of t...
Carbohydrate-Mediated Biomolecular Recognition and Gating of Synthetic Ion Channels
2013
Nanochannel-based biosensing devices have been proposed for selective detection of protein analyte molecules. However, the design and miniaturization of reusable channel-based biosensors is still a challenge in nanoscience and biotechnology. We present here a reusable nanofluidic biosensor based on reversible lectin-carbohydrate interactions. The nanochannels are fabricated in heavy ion tracked polymer membranes. The channel walls are functionalized with p-aminophenyl alpha-D-mannopyranoside (APMP) monolayers through carbodiimide coupling chemistry. The chemical (mannopyranoside) groups on the inner channel walls serve as binding sites and interact with specific protein molecules. The bindi…
Testing the recoverability of grass DNA transferred to textiles for forensic purpose
2010
Botanical evidence such as grass stains on textiles is sometimes present in the crime scene and can allow investigators to establish an association between persons linked to the criminal event and the crime scene. In this study, extraction of grass DNA from stains on textiles was undertaken. DNA extraction was performed on four grass species conserved both indoors and outdoors for 7, 14 and 30 days after staining. Once the extracted DNA was quantified, a polymerase chain reaction (PCR) amplifying a fragment of the internal transcribed spacer was performed.DNA extraction was successful in 97.5% of samples. No significant differences in the amount of extracted DNA were detected among species …
Complementary methods assessing short and long-term prey of a marine top predator ‒ Application to the grey seal-fishery conflict in the Baltic Sea.
2019
The growing grey seal (Halichoerus grypus) population in the Baltic Sea has created conflicts with local fisheries, comparable to similar emerging problems worldwide. Adequate information on the foraging habits is a requirement for responsible management of the seal population. We investigated the applicability of available dietary assessment methods by comparing morphological analysis and DNA metabarcoding of gut contents (short-term diet; n = 129/125 seals, respectively), and tissue chemical markers i.e. fatty acid (FA) profiles of blubber and stable isotopes (SIs) of liver and muscle (mid- or long-term diet; n = 108 seals for the FA and SI markers). The methods provided complementary inf…
Infection of the upper extremity by Mycobacterium marinum in a 3-year-old boy--diagnosis by 16S-rDNA analysis.
1996
A 3-year-old boy developed several subcutaneous nodular lesions on his right arm. Based on the histological examination of one of these nodules furunculosis was suspected and cefuroxime was tentatively given. However, acid-fast bacilli were then detected in the tissue specimen and a few colonies of acid fast, gram-positive rods grew on blood agar. Definitive species diagnosis (Mycobacterium marinum) was rapidly achieved by automated sequencing of amplified 16S-rDNA and antimicrobial therapy was adjusted according to the available literature. After 3 weeks of treatment with clarithromycin, rifampicin and protionamid regression of the nodular lesions was evident.